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KMID : 0364320140390030009
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Journal of East-West Medicine
2014 Volume.39 No. 3 p.9 ~ p.19
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Protective Effect of Sauchinone against Liver Toxicity Induced by Tacrine
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Han Nu-Ri
Park Sang-Mi
Byun Sung-Hui
Cho Il-Je
Kim Young-Woo
Kim Sang-Chan
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Abstract
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Objectives: Sauchinone is a lignan isolated from Saururus chinensis which is a traditional herb in Korea. Tacrine, the cholinesterase inhibitor, had been used as a drug for treating Alzheimer disease. However, therapeutic application of tacrine is restricted because of hepatotoxicity. This study investigated whether sauchinone can protect hepatocytes against the mitochondrial dysfunction and apoptosis caused by tacrine.
Methods: We used HepG2 cells, the human-derived hepatocyte cell line, to determine sauchinone prevents tacrine-induced hepatic toxicity. After HepG2 cells were treated with sauchinone for 12 h, the cells were subsequently exposed to tacrine. Effects of sauchinone against tacrine-induced cell damage were examined by fluorescence activated cell sorter and immunoblot analysis and so on.
Results: Pre-treatment of sauchinone inhibited cell death caused by tacrine in a dose dependent manner, maintaining the transition of mitochondria membrane potential. In addition, sauchinone prevented oxidative stress induced by tacrine.
Conclusion: Sauchinone protects hepatocytes and mitochondria exposed to toxicity of tacrine. These results suggest that sauchinone may be a new therapeutic candidate for supplementing tacrine.
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KEYWORD
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Sauchinone, Tacrine, Iiver, toxicity
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